Rapid Coomassie Blue Staining and Destaining of Polyacrylamide Gels

Rapid Coomassie blue staining and destaining of polyacrylamide gels.

Heating greatly speeds Coomassie blue staining and destaining.

All loading buffers allowed the target amplification at 0.25 and 0.5 ×final concentration (Figure 2). However, at 0.5 × final concentration , there was a decrease in the amplification that was more pronounced than that observed with the 359 bp target depicted in Figure 1. After the 0.25 ×gel-loading buffers in the PCR mixture proved to be feasible even when the bacterial lysate was diluted 1000...

Detergent-mediated destaining of coomassie brilliant blue-stained SDS polyacrylamide gels.

A simple and one-step detergent-mediated destaining procedure for SDS Polyacrylamide gels for proteins is described. Suspension (5%, w/v) of a commercially available household detergent, Vim Ultra, has been found to be very efficient in destaining polyacrylamide gels without interfering with the resolution of proteins. As compared to the routinely used solvent (methanol-acetic acid-water)-media...

Removal of coomassie blue precipitates from polyacrylamide gels.

During staining of polyacrylamide gels, varying amounts of Coomassie blue may precipitate on the surface of the gels. This background obscures faint bands and is esthetically unappealing. The problem is worse when gels are stained for longer periods of time or when the stain has not been recently filtered. Rinsing a gel with methanol efficiently and quickly removes this background. Basically, a...

Fast and sensitive colloidal coomassie G-250 staining for proteins in polyacrylamide gels.

Coomassie Brilliant Blue (CBB) is a dye commonly used for the visualization of proteins separated by SDS-PAGE, offering a simple staining procedure and high quantitation. Furthermore, it is completely compatible with mass spectrometric protein identification. But despite these advantages, CBB is regarded to be less sensitive than silver or fluorescence stainings and therefore rarely used for th...